Top

1. What's new

2. Preface

3. Backgrounds

3.1 What is FRET?
3.2 How to use

4. What's Phogemon?

4.1 Basic structure
4.2 FP
4.3 Sensor and ligand
4.4 Spacer
4.5 Localization
4.6 Cells
4.7 Development

5. Phogemons

5.1 Small GTPases
5.2 Kinases
5.3 PtdInss
5.4 Data sheet

6. Analysis

6.1 Basic method
6.2 Validation
6.3 Advanced method

7.FRET Imaging

7.1 Dark room
7.2 Microscope
7.3 Light source
7.4 DIC and PH
7.5 Chamber
7.6 CCD camera
7.7 Filter set
7.8 Analysis
7.9 Technical tips

8 Examples

8.1 Ras
8.2 Tyrosine kinase

9. References

10. Acknowledgement

11. Copyright