Lecturer: Michiyuki Matsuda, M.D., Ph.D., Kenta Terai, M.D., Ph.D., Takeshi Hirashima, Ph.D.

In this training course, a pair of students will learn the basic principles of fluorescence imaging and practice how to use fluorescence microscopes.

Agenda

Date	Content
March 5, 2018 (Mon)	Lecture on fluorescence microscopy Preparation of cells for imaging
March 6, 2018 (Tue)	Fluorescence live imaging of tissue culture cells #1
March 7, 2018 (Wed)	Fluorescence live imaging of tissue culture cells #2 Digital image analysis #1
March 8, 2018 (Thr)	Digital image analysis #2 Statistical analysis of imaging data
March 9, 2018 (Fri)	Discussion Preparation of figures
March 12, 2018 (Mon)	Lecture on in vivo imaging of mice How to use two-photon excitation microscopes
March 13, 2018 (Tue)	Preparation of immune cells from mice Ex vivo imaging of retina
March 14, 2018 (Wed)	In vivo imaging of cancer cells
March 15, 2018 (Thr)	Discussion Preparation of figures
March 16, 2018 (Fri)	Discussion Preparation of presentation slides