The Wild & Wise (W&W) Biostudies Workshop

The Bioimaging Training Course at Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies

Lecturer: Michiyuki Matsuda, M.D., Ph.D., Kenta Terai, M.D., Ph.D., Takeshi Hirashima, Ph.D.

In this training course, a pair of students will learn the basic principles of fluorescence imaging and practice how to use fluorescence microscopes.

Agenda

Date Content
March 5, 2018 (Mon)
  • Lecture on fluorescence microscopy
  • Preparation of cells for imaging
March 6, 2018 (Tue)
  • Fluorescence live imaging of tissue culture cells #1
March 7, 2018 (Wed)
  • Fluorescence live imaging of tissue culture cells #2
  • Digital image analysis #1
March 8, 2018 (Thr)
  • Digital image analysis #2
  • Statistical analysis of imaging data
March 9, 2018 (Fri)
  • Discussion
  • Preparation of figures
March 12, 2018 (Mon)
  • Lecture on in vivo imaging of mice
  • How to use two-photon excitation microscopes
March 13, 2018 (Tue)
  • Preparation of immune cells from mice
  • Ex vivo imaging of retina
March 14, 2018 (Wed)
  • In vivo imaging of cancer cells
March 15, 2018 (Thr)
  • Discussion
  • Preparation of figures
March 16, 2018 (Fri)
  • Discussion
  • Preparation of presentation slides
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