|
Targets |
Composition |
Name |
Lab |
PubMed |
Year |
| Tyrosine kinase, substrate phosphorylation |
|
|
EGFR |
CFP-SH2 (Shc)-Substrate (synthetic)-YFP |
EGFR-indicator |
Tsien |
11752449 |
2001 |
|
EGFR |
EGFR-CFP-PTB (Shc)-YFP |
FLAME |
Basitiaens |
15215236 |
2004 |
|
EGFR |
α-helix-YFP-CrkII-CFP |
Picchu-Z |
Matsuda |
15922734 |
2005 |
|
Insulin receptor |
CFP-Substrate (IRS1)-SH2 (p85)-YFP |
Phocus |
Umezawa |
11875431 |
2002 |
|
Abl, EGFR |
YFP-CrkII-CFP |
Picchu |
Matsuda |
11406630 |
2001 |
|
Abl, EGFR |
YPet-CrkII-EV-SECFP
|
PicchuEV |
Matsuda |
21976697 |
2011 |
|
Abl, EGFR |
CFP-CrkII-YFP |
Abl indicator |
Tsien |
11752449 |
2001 |
|
Abl |
Venus-CrkL-ECFP |
Pickles |
Ohba |
20670950 |
2010 |
|
Src |
TM (Cbp)-YFP-Substrate (Cbp)-SH2 (Csk)-CFP |
Chimera |
Okada |
11880369 |
2003 |
|
Src |
CFP-SH2 (Src)- Substrate (synthetic)-YFP |
Src indicator |
Tsien |
11752449 |
2001 |
|
Src |
CFP-SH2 (Src)- Substrate (synthetic)-YFP |
Src reporter |
Wang Y |
15846350 |
2005 |
|
Src |
sfGFP-SH2 (Src)- Substrate (synthetic)-mTagBFP |
BG Src biosensor |
Zhang Z |
23517824 |
2013 |
|
SYK |
ECFP-SH2 (SYK)- linker -Substrate-YPet |
SYK biosensor |
Zhu C |
25541586 |
2011 |
| |
ZAP70 |
mCFP-SH2 (Grb2)- linker -Substrate-YPet |
ROSA-XL |
Lellouch |
25735979 |
2015 |
| Tyrosine kinase, conformational change |
|
|
FAK |
CFP-FERM-Citrine-kinase-FAT |
CYFAK413 |
Schaller |
17967873 |
2008 |
|
FAK |
YFP-FERM-CFP-KD-FAT |
FERM-sensor |
Bunt |
19208768 |
2009 |
|
FAK |
mCitrineFERM-TEV-protease site-ER/K-linke-mCerulean-kinase and FAT domains |
SPASM sensor |
Sivaramakrishnan |
23393139 |
2009 |
| Serine/threonine kinase, substrate phosphorylation |
|
| AGC family |
|
PKA |
GFP-KID (PKA)-BFP |
ART |
Hagiwara |
10700148 |
2000 |
|
PKA |
CFP-[14-3-3]-Substrate (synthetic)-YFP |
AKAR |
Tsien |
11752448 |
2001 |
|
PKA |
Ypet-FHA1-EV-Substrate-ECFP |
AKAR3-EV |
Matsuda |
21976697 |
2011 |
|
S6K |
YPet-FHA1-EV-Substrate-Turquiose |
Eevee-S6K |
Matsuda |
21976697 |
2011 |
|
RSK |
YPet-FHA1-EV-Substrate-ECFP |
Eevee-RSK |
Matsuda |
21976697 |
2011 |
|
Aurora B |
CFP-FHA2-substrate(Kif2 57-70)-YFP |
Aurora B sensor |
Kapoor |
18463638 |
2008 |
|
Plk-1 |
CFP-FHA2-substrate(Myt1 421-432)-YFP |
Plk sensor |
Medema |
18615013 |
2008 |
|
PKC |
CFP-FHA2 (Rad53P)-Substrate(synthetic)-YFP |
CKAR |
Newton |
12782683 |
2003 |
|
PKC |
YFP-PH and DEP (pleckstrin)-GFP |
KCP-2 |
Schultz |
16390103 |
2006 |
|
PKC |
YFP-PH and DEP (pleckstrin)-GFP |
KCAP-1 |
Schultz |
16390103 |
2006 |
|
PKC |
C1-YPet-FHA1-EV-Substrate-ECFP |
Eevee-PKC |
Matsuda |
21976697 |
2011 |
|
AKT/PKB |
CFP-[14-3-3]-substrate-YFP |
Aktus |
Umezawa |
12773546 |
2003 |
|
AKT/PKB |
CFP-FHA2-substrate-YFP |
BKAR |
Newton |
15583002 |
2005 |
|
Akt/PKB |
PH-YPet-FHA1-EV-Substrate-ECFP |
Eevee-Akt |
Matsuda |
21976697 |
2011 |
|
Akt/PKB |
PH-YPet-FHA1-EV-Substrate-ECFP |
Eevee-iAkt |
Aoki |
24212374 |
2014 |
|
Rho kinase/ROCK |
Ypet-FHA1-EV-Substrate-ECFP |
Eevee-ROCK |
Matsuda |
27885213 |
2016 |
| CMGC family |
|
ERK |
CFP-FHA2-substrate-YFP-D domain |
Erkus |
Umezawa |
17261026 |
2007 |
|
ERK |
mRFP-WW-Long linker-substrate-D domain-EGFP |
EKAR |
Sbovoda |
19033456 |
2008 |
|
ERK |
Ypet-WW-EV-Substrate-D domain-ECFP |
EKAREV |
Matsuda |
21976697 |
2011 |
|
ERK |
mTurquoise-WW-EV-Substrate-domain-cp175-Venus-Venus |
EKAREV-TVV |
Bodart JF |
24434874 |
2014 |
|
JNK |
ECFP-FHA1-JDP2-JDP2delta-Citrine |
JNKAR1 |
Zhang |
20212108 | 2010 |
|
JNK |
YPet-FHA1-EV-JDP2-JDP2delta-ECFP |
JNKAR1EV |
Matsuda |
21976697 |
2011 |
|
p38 |
YPet-WW-Substrate-SECFP |
PerKy-381 |
Saito H |
26399197 |
2015 |
| STE family |
|
SAP3K |
YFP-FHA1-MKK6 DN- DVD domain-CFP |
SAP3K reporter |
Saito |
19737916 |
2009 |
| CAMK family |
|
MLCK, Rho kinase |
CFP-Myosin light chain-YFP |
CRCit |
Iino |
19033456 |
2005 |
|
AMPK |
ECFP-FHA1-substrate-Venus |
AMPKAR |
Cantley |
21459332 |
2011 |
|
AMPK |
ECFP-FHA1-substrate-cpV-E172 |
ABKAR |
Zhang |
25788287 |
2015 |
|
PKD |
CFP-FHA2-substrate-YFP |
DKAR |
Newton |
17189263 |
2007 |
| TKL |
|
Tak1 |
YPet-FHA1-EV-substrate-ECFP |
Eevee-Tak1 |
Matsuda |
26931406 |
2007 |
| atypical PK |
|
ATM |
YFP-Chk2_substrate-FHA1-CFP |
ATM reporter |
Hunter |
17428747 |
2007 |
| Serine/threonine phosphatases, substrate type |
|
|
Calcineurin (PP2B) |
CFP-NFAT-Venus |
CaNAR1 |
Zhang |
18493642 |
2008 |
|
CDC25/Plk1 |
mTurquoise-FHA2-Cdc25-Ypet |
Cdc25C-S75 biosensor |
Gavet O |
26254922 |
2016 |
| Serine/threonine kinase, conformational change |
|
|
AKT/PKB |
GFP-AKT-YFPdark |
GFP-AKT-YFP |
Larijani |
12662152 |
2003 |
|
AKT/PKB |
PH (Akt)-YFP-AKT-CFP |
Akind |
Matsuda |
17079732 |
2007 |
|
PKCdelta |
CFP-PKC-YFP |
CY-PKCdelta |
Blumberg |
15611119 |
2005 |
|
c-Raf |
YFP-cRaf-CFP |
Prin-cRaf |
Matsuda |
15711535 |
2005 |
|
B-Raf |
YFP-BRaf-CFP |
Prin-Braf |
Matsuda |
16858395 |
2006 |
|
ERK |
YFP-ERK-CFP |
Miu2 |
Matsuda |
16418172 |
2006 |
|
MLCK |
MLCK-GFP-BFP |
MLCK-FIP |
Chisholm |
11815633 |
2002 |
|
MLCK |
MLCK-CFP-YFP |
MLCK-FIP |
Chew |
20067998 |
2010 |
|
DAPK1 |
mTurquoise-DAPK1-Venus |
DAPK1(334)-F40 |
Schultz |
20067998 |
2011 |
|
CAMK2a |
mTurquoise-Camk2a-Venus |
Camk2a(314)-F40 |
Schultz |
20067998 |
2011 |
|
CAMK2a |
mTurquoise-Camk2a-Venus |
Camk2a-F63 |
Schultz |
20067998 |
2011 |
|
CAMK2a |
YFP-Camk2a-CFP |
Camui-alpha |
Hayashi |
20067998 |
2005 |
| Histon methylation |
|
|
Histon H3-K9, K27 |
CFP-Chromodomain-histonH3-YFP |
K9, K27 reporter |
Ting |
15137760 |
2004 |
|
Histon H4-K5, K8 |
Venus-BRDT bromodomain-linker-HistoneH4-CFP |
Histac |
Yoshida |
19805290 |
2009 |
| Small GTPases |
|
|
Ras, Rap1 |
YFP-Ras-Raf-CFP |
Raichu-Ras, Rap1 |
Matsuda |
11429608 |
2001 |
|
Ras |
Ypet-Ras-EV-Raf-TurquoiseGL |
RaichuEV-Ras |
Matsuda |
21976697 |
2011 |
|
Ras, Rap1 |
RasRBD (Byr2)-Cerulean3-Venus-HRas |
DORA-Ras |
Mayer |
25043303 |
2015 |
|
Ral |
YFP-Ral-RalBP1-CFP |
Raichu-RalA, RalB |
Matsuda |
15034142 |
2004 |
|
RRas |
YFP-Rras-RalGDS-CFP |
Raichu-RRas |
Matsuda |
17344481 |
2007 |
|
RhoA |
YFP-PKN-CFP |
Raichu-RhoA |
Matsuda |
12860967 |
2003 |
|
RhoA |
RBD-CFP-YFP-RhoA |
RhoA biosensor |
Hahn |
16547516 |
2006 |
|
RhoA |
YFP-Rhotekin-CFP |
Raichu-RBD |
Matsuda |
12860967 |
2003 |
|
Rac1 |
YFP-PAK-Rac1-CFP |
Raichu-Rac1 |
Matsuda |
12192056 |
2002 |
|
Rac, Cdc42 |
YFP-PAK-CFP |
Raichu-CRIB |
Matsuda |
12192056 |
2002 |
|
Cdc42 |
YFP-PAK-Cdc42-CFP |
Raichu-Cdc42 |
Matsuda |
12192056 |
2002 |
|
Rac, Cdc42 |
YPet-PAK-Rac1/Cdc42-CFP |
Raichu-Rac |
Wang |
18799748 |
2008 |
|
Rac, Cdc42 |
YPet-PAK-EV-Rac1-CFP |
Raichu-Rac |
Matsuda |
21976697 |
2011 |
|
Cdc42 |
Cdc42-YFP-GBD-ACV-CFP |
GEF sensor |
Rosen |
12680752 |
2003 |
|
N-WASP/Cdc42 |
CFP-N-WASP-YFP |
N-WASP-BS |
Condeelis |
15084285 |
2004 |
|
Cdc42 |
mCer-PAK_PBD- cp229Venus-Cdc42 |
Cdc42 biosensor |
Hodgson L |
24798463 |
2014 |
|
TC10 |
YFP-POSH-TC10-CFP |
Raichu-TC10 |
Matsuda |
16950130 |
2006 |
|
Rab5 |
YFP-EEA1-CFP-Rab5 |
Raichu-Rab5 |
Matsuda |
18385674 |
2008 |
| GPCR |
|
|
PTHR |
PTHR-CFP-PTHR-YFP |
PTHR-cam |
Lohse |
12808462 |
2003 |
|
2AAR |
2AAR-CFP-2AAR-YFP |
2AAR-cam |
Lohse |
12808462 |
2003 |
|
Orexin receptor |
OX-Flash-OX-CFP |
OX1 FRET sensor |
Xu TR |
22389503 |
2012 |
|
DHPR |
Gb-YFP-G |
β1a reporter |
Balijepalli RC |
26114725 |
2015 |
| Lipids |
|
|
PIP3 |
CFP-PH (GRP)-YFP-membrane anchor |
Fllip/Pippi-PIP3 |
Umezawa |
14528311 |
2003 |
|
PIP2 and PIP3 |
CFP-ActA-YFP-membrane anchor |
CAY |
Cicchetti |
14967034 |
2003 |
|
PIP3, PI(3,4)P2 |
CFP-PH(Akt)-Pseudoligand-YFP |
InPAkt |
Zhang |
16214892 |
2005 |
|
PI(4,5)P2 |
CFP-PH (GRP)-YFP-membrane anchor |
CYPHR |
Newton |
12782683 |
2003 |
|
PI(4,5)P2 |
CFP-PH (PLCd)-YFP-membrane anchor |
Pippi-PI(4,5)P2 |
Matsuda |
18685081 |
2008 |
|
PI(3,4)P2 |
CFP-PH (TAPP1)-YFP-membrane anchor |
Pippi-PI(3,4)P2 |
Matsuda |
17079732 |
2007 |
|
IP3 |
CFP-IP3 receptor-YFP |
LIBRA |
Tojyo |
15272011 |
2004 |
|
IP3 |
CFP-IP3 receptor-YFP |
fretino |
Umezawa |
16053285 |
2005 |
|
IP3 |
CFP-IP3 receptor-YFP |
FIRE |
Mignery |
16249182 |
2006 |
|
Diacylglycerol |
CFP-C1 (PKC)-YFP |
DAGR |
Newton |
12782683 |
2003 |
|
Diacylglycerol |
CFP-CRD (PKC)-YFP-membrane anchor |
Daglas |
Umezawa |
16990811 |
2006 |
|
Diacylglycerol |
CFP-CRD (PKC)-YFP-membrane anchor |
DIGDA |
Matsuda |
18685081 |
2008 |
|
PI(4)P |
CFP-PH (GRP)-YFP-membrane anchor |
Pippi-PI(4)P |
Matsuda |
18685081 |
2008 |
|
Phosphatidic acid |
CFP-PA binding doman-YFP-membrane anchor |
Pii |
Matsuda |
20826779 |
2010 |
| Ions |
|
|
calcium |
CFP-Calmodulin-M13-YFP |
cameleon |
Tsien |
9278050 |
1997 |
|
|
Other calcium sensors: Miyawaki and Niino |
|
|
|
2015 |
|
Zn |
Cerulean-Atox1-WD4-Citrine |
eCALWY-1 |
Merkx M |
19718032 |
2009 |
|
Zn |
Cerulean-HsCen2-Citrine |
MagFRET |
Merkx M |
24312622 |
2013 |
| Voltage |
|
|
K+ channel |
Kv potassium channel-CFP-YFP |
VSFP1 |
Knoepfel |
11454036 |
2001 |
|
|
Other voltage sensors: Lin and Schnitzer |
|
|
|
2016 |
| Steroids |
|
|
androgen |
CFP-androgen binding domain-coactivator-YFP |
ficaro |
Umezawa |
16555356 |
2006 |
|
estrogen/androgen |
CFP-estrogen or androgen receptor-YFP |
CEY, CAY |
Lannigan |
15985367 |
2005 |
|
retinoic Acid |
CFP-RAR-YFP |
GEPRAs |
Miyawaki |
23563268 |
2013 |
| Amino acids |
|
|
glutamate |
CFP-glutamate binding domain-YFP |
FLIPE |
Frommer |
15939876 |
2005 |
|
glutamate |
ECFP-glutamate binding domain-Citrine |
GluSnFR |
Tsien |
18332427 |
2008 |
|
l-arginine |
ECFP-AncQ-Venus |
cpFLIPR |
Jackson |
26061224 |
2015 |
|
Citrate |
CFP-CitA-Venus |
CitA |
Zamboni |
3229521 |
2011 |
| Sugars |
|
|
sugar |
CFP-sugar binding domain-YFP |
nanosensors |
Frommer |
15667328 |
2005 |
|
sucrose |
eCFP-AtThuE -eYFP |
FLIPsuc |
Frommer |
16912038 |
2006 |
|
glucose |
MglB(1-12)-ECFP-MglB (13-304)-linker-Citrine |
FLII12Pglu |
Frommer |
18177733 |
2007 |
|
maltose |
ECFP-MBP-EYFP |
FLIPmal |
Frommer |
12097642 |
2002 |
|
lactate |
mTFP-LidR-Venus |
Laconic |
Barros |
23469056 |
2013 |
|
Sucrose, Trehalose |
eCFP-AtThuE-mVenus |
FLIPsuc90μΔ1Venus |
Kikawada |
26214383 |
2015 |
| Nucleotides |
|
|
|
|
|
|
cGMP |
YFP-PKG-CFP |
CGY |
Umezawa |
11140757 |
2000 |
|
cGMP |
CFP-GKI-YFP |
cygnet |
Dostmann |
11226257 |
2001 |
|
cGMP |
YFP-phosphodiesterase-CFP |
pGES-DE2 |
Lohse |
16369548 |
2006 |
|
cGMP |
T-Sappphire-PDE5-dimer2 |
red cGES-DE5 |
Oka |
19551140 |
2009 |
|
cAMP |
CFP-Epac-YFP |
CFP-Epac-YFP |
Jalink |
15550931 |
2004 |
|
cAMP |
CFP-Epac-YFP |
ICUE |
Zhang |
15545605 |
2004 |
|
cAMP |
CFP-Epac-YFP |
Epac2-camps |
Lohse |
15231839 |
2004 |
|
cAMP |
ECFP-Epac-Citrine |
Epac2-camps300-Cit |
Vincent |
20427672 |
2010 |
|
cAMP |
mTurquoise-Epac-cp173Venus-Venus |
TEpacVV |
Jalink |
21559477 |
2011 |
|
cAMP |
ECFP-cAMP-binding domain B
(mammalian PKA RII beta-subunit)-YFP |
cAMP FRET sensor |
Parent |
19808889 |
2009 |
|
ATP |
mseCFP-eSubunit(bacterial FoF1-ATP synthase)-cp173mVenus |
ATeam |
Noji |
19720993 |
2009 |
|
ATP |
mKO-eSubunit(bacterial FoF1-ATP synthase)-cp173mVenus |
GO-ATeam |
Noji |
21488691 |
2009 |
| Proteases |
|
|
Caspase3, 6 |
CFP-SGDEVD-YFP-RSRAQASVEID-mRFP |
CFP-c3-YFP-c6-mRFP |
He |
16683263 |
2006 |
|
Caspase8 |
YFP-Bid-CFP |
CFP-c3-YFP-c6-mRFP |
Taira |
12409609 |
2002 |
|
Caspase3, 9 |
ECFP-cleavage site (DEVD or VEID) -Venus |
SCAT3, 9 |
Miura |
12527749 |
2003 |
|
Caspase3 |
BFP-DEVD-GFP |
BFP-DEVD-GFP |
Prehn |
11964393 |
2002 |
|
Caspase3 |
CFP-DEVD-YFP |
CFP-DEVD-YFP |
Tavare |
11256610 |
2000 |
|
Caspase3 |
EYFP-DEVD-EGFP |
EYFP-DEVD-EGFP |
Bastiaens |
11175733 |
2001 |
|
Caspase3 |
EGFP-DEVD-EBFP |
EGFP-DEVD-EBFP |
Luo |
9518501 |
1998 |
|
Caspase3 |
mAmetrine-DEVD-tdTomato, mCitrine-DEVD-mTFP1 |
mAmetrine-DEVD-tdCaspase3 biosensor |
Campbell |
18425137 |
2008 |
|
MMP activity |
Igk-Chain-YFP-MSS-CFP-TM (PDGFR) |
YFP-MSS-CFPdisplay |
Luo |
17187878 |
2007 |
|
MMP activity |
6His-YFP-MSS-CFP |
YFP-MSS-CFPdisplay |
Luo |
17187878 |
2007 |
|
MT1-MMP |
Ypet-[MMP substrate]-CFP |
MT1-MMP FRET biosensor |
Wang |
18441011 |
2008 |
|
MT1-MMP |
mCherry-MT1-[MMP substrate]-mOrange2 |
MT1-MMP FRET biosensor |
Wang |
20197470 |
2010 |
|
MMP-9 |
Signal peptide-Venus2-linker-Venus1-[MMP9 substrate]-linker-mTFP1-PDGFR |
MMP-9 activity biosensor |
Wlodarczyk J |
24290700 |
2013 |
|
m-caplain activity |
EYFP-mCalpain cleavage site-ECFP-PDZ1-1 targeting seq |
YSCS |
Edelman |
10688895 |
2000 |
|
ADAM17 |
TGFα-YPet-TNFα cleavage site-eCFP-PDGFR |
TSen |
Liu X |
25714465 |
2015 |
| Other signaling molecules |
|
|
HIV Rev |
YFP-Rev peptide-CFP |
YRFnC-11ad |
Kobatake |
16013840 |
2005 |
|
Presenilin (PS) |
GFP-PS1-RFP |
GFP-PS1-RFP |
Luo |
19924286 |
2009 |
|
Plasma membrane Ca(2+) pump |
BFP-plasma membrane Ca2-pump-GFP |
BFP-PMCA-GFP |
Adamo |
17901055 |
2007 |
|
LuxP |
CFP-LuxP (BAI-2 receptor)-YFP |
CLPY |
Sayre |
17352493 |
2007 |
|
MyosinII |
YFP−myosin(motor domain)−CFP |
YFP-myosin-CFP |
Bagshaw |
16939200 |
2006 |
|
MyosinII |
GFP−myosin(motor domain)−BFP |
GFP-myosin-BFP |
Bagshaw |
16939200 |
2006 |
|
MyosinII |
His-GFP−S1dC (motor domain of MyosinII)−BFP |
chimaera |
Sutoh |
9845076 |
1997 |
|
MyosinII |
His-BFP−S1dC−GFP |
chimaera |
Sutoh |
9845076 |
1997 |
|
REDOX |
Cerulean-Yap1 c-CRD-Citrine |
Redoxfluor |
Sakai |
20498274 |
2010 |
|
REDOX |
ECFP-RL7-EYFP |
redox biosensor |
Gaskins |
21606117 |
2011 |
|
Pyruvate kinase |
Citrine-Pyruvate kinae-Cerulean |
PKAR |
Satin S |
24100037 |
2013 |
|
Pyruvate kinase |
Cerulean-1.3C-Citrinen |
redox sensor |
Karp M |
25224640 |
2014 |
|
Vinculin |
CFP-Vinculin-YFP |
vinculin activation biosensor |
Craig S |
15883197 |
2005 |
|
Vinculin |
Vh-mTFP-elastic linker-Venus-Vt |
vinculin tension sensor |
Schwartz M |
20613844 |
2010 |
|
|
|
|
|
|
|
|