Summary of FRET-based monitor
Signal transduction monitors based on GFPand FRET |
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Target |
Structure |
Ref |
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Receptors/tyrosine kinases |
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|
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|
EGFR |
2 |
EGFR-GFP |
anti-PY-Cy3 |
(1) |
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|
EGFR |
2 |
EGFP-CFP |
Grb2-YFP |
(2) |
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|
Abl/EGF-R |
1 |
YFP-CrkII-CFP (Picchu) |
(3) |
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|
Abl |
1 |
CFP-CrkII-YFP |
(4) |
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|
Insulin R |
1 |
CFP-substrate-SH2-YFP (Phocus) |
(5) |
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|
Src |
1 |
TM(Cbp)-YFP-Cbp(substrate)-SH2 (Csk)-CFP |
(6) |
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|
Src |
1 |
CFP-SH2-substrate-YFP |
(4) |
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|
|
|
|
|
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Serine/threonine kinases |
|
|
|
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|
Raf |
1 |
YFP-Raf-CFP |
(7) |
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|
PKA |
1 |
GFP-KID-BFP |
|
(8) |
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|
PKA |
1 |
CFP-14.3.3-substrate-YFP (AKAR) |
(9) |
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|
PKA |
1 |
RGFP-substrate-BGFP (ART) |
(10) |
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|
AKT/PKB |
1 |
CFP-14.3.3-substrate-YFP [Akt Phocus (Aktus)] |
(11) |
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|
AKT/PKB |
1 |
GFP-AKT-red shifted YFP |
(12) |
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|
PKC |
2 |
PKCa-GFP |
phosphospecific Ab-Cy3, 5 |
(13) |
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|
PKC |
1 |
CFP-FHA2(Rad53P)-substrate-YFP (CKAR) |
(14) |
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|
|
|
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Small GTPases |
|
|
|
|
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|
Ras |
1 |
YFP-Ras-Raf-RBD-CFP (Raichu-Ras) |
(15) |
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|
Rap1 |
1 |
YFP-Ras-Raf-RBD-CFP (Raichu-Rap1) |
(15) |
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|
Ras |
2 |
Raf-RBD-CFP |
CD8-YFP |
(16) |
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|
RalA |
1 |
YFP-RalA-RalBP1-RBD-CFP |
(17) |
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|
RhoA |
1 |
YFP-RhoA-PKN-RBD-CFP (Raichu-Rho) |
(18) |
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|
RhoA |
1.5 |
YFP- RBD-CFP |
(18) |
|||
|
Rac |
1 |
YFP-PAK-CRIB-Rac-CFP (Raichu-Rac) |
(19) |
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|
Rac |
2 |
GFP-Rac |
CRIB-Alexa546 |
(20) |
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|
Cdc42 |
1 |
YFP-RAK-CRIB-Cdc42-CFP (Raichu-Cdc42) |
(19) |
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|
Rac, Cdc42 |
1.5 |
YFP-CRIB-CFP (Raichu-CRIB) |
(19) |
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|
Cdc42 |
1 |
YFP-GBD-ACV (N-WASP)-CFP |
(21) |
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|
Cdc42 |
1.5 |
YFP-GBD-CFP |
(21) |
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|
Cdc42 |
1 |
Cdc42-YFP-GBD-ACV-CFP (GEF sensor) |
(21) |
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