FRET Mice at Matsuda Lab@Kyoto University

May 27, 2019

All mice are generated by Kenta Sumiyama@QBiC except ROSA26-targed mice developed by Takaya Abe@CDB.

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Target Alias Transgene Strain ID Tissue Reference etc.
Protein kinases
ERK Eisuke EKAREV-NES B6-Tg(EKAREV)pT2A-3903NES nbio_183 Ubiquitous Kamioka 2012; Mizuno 2014; Okuchi 2016; Sano 2016; Yamauchi 2016; Hiratsuka 2017; Ihermann-Hella 2018; Muta 2018; Sano 2018; Genotyping
ERK Balb/c.Eisuke-NES EKAREV-NES Balb/c-Tg(EKAREV)pT2A-3903NES nbio_284 Ubiquitous
ERK hyBRET-Eisuke hyBRET-ERK-NES B6 Albino-Tg(hyBRET-ERK-NES)pT2A-6011NES nbio_325 Ubiquitous Komatsu 2018 ; Imanishi 2018
ERK R26R-EKAREV-NES EKAREV-NES Gt(ROSA)26Sortm1(CAG-loxP-tdKeima-loxP-EKAREV-NES) CDB0306K Cre-inducible Konishi 2018
ERK ERK-NES-B6 Albino EKAREV-NES B6 Albino-Tg(EKAREV)pT2A-3903NES nbio_332 Ubiquitous
ERK lox-Eisuke EKAREV-NES B6-Tg(EKAREV)pT2A-lox-tdKeima-3903NES nbio_211 Cre-inducible Goto 2015; Yamaguchi 2015
ERK FVB.lox-Eisuke-NES EKAREV-NES FVB.Tg(lox-EKAREV)pT2A-lox-tdKeima-3903NES nbio_274 Cre-inducible
ERK lox-HyBRET-ERK-NES hyBRET-EKAREV-NES B6 Albino-Tg(loxP-mCherry-NES-loxP-hyBRET-EKAREV-NES) Cre-inducible
ERK Eisuke-NLS EKAREV-NLS B6-Tg(EKAREV)pT2A-3905NLS nbio_184 Ubiquitous Kamioka 2012
ERK hyBRET-Eisuke-NLS hyBRET-ERK-NLS B6 Albino-Tg(hyBRET-ERK-NLS)pT2A-6011NLS nbio_326 Ubiquitous Komatsu 2018Kinjo 2019
ERK R26R-EKAREV-NLS EKAREV-NLS Gt(ROSA)26Sortm1(CAG-loxP-tdKeima-loxP-EKAREV-NLS) CDB0307K Cre-inducible Konishi 2018
ERK FVB.Eisuke-NLS EKAREV-NLS FVB-Tg(EKAREV)pT2A-3905NLS(N6) nbio_255 Ubiquitous Kamioka 2012; Hiratsuka 2015; Aoki 2017
ERK/ Calcium lox-hyBRET-ERK-NLS-RGECO lox-tdKeima-NES-stop-lox-6011nls-P2Av3-RGECO1 B6 Albino-Tg(loxP-tdKeima-NES-stop-loxP-6011nls-P2Av3-RGECO1) Cre-inducible
ERK lox-HyBRET-ERK-NLS hyBRET-EKAREV-NLS B6 Albino-Tg(loxP-mCherry-NES-loxP-hyBRET-EKAREV-NLS) Cre-inducible
ERK ERK-NLS-B6 Albino EKAREV-NLS B6 Albino-Tg(EKAREV)pT2A-3905NLS nbio_333 Ubiquitous
JNK Junko JNKAR1EV ICR~(ICR~B6C3F1)N2- Tg(pT2A-3907NLS) nbio_189 Ubiquitous Goto 2013
ATM Bruce Eevee-ATM B6C3F1-Tg(pT2A-lox-tdKeima-FHA1-TQCHK2subs) nbio_189 Cre-inducible
PKA PKAchu AKAREV B6-Tg(AKAR3EV)pT2A-3901NES nbio_185 Ubiquitous Kamioka 2012; Mizuno 2014; Okuchi 2016; Sano 2016; Yamauchi 2016; Hiratsuka 2017; Sato 2020; Genotyping
PKA Balb/c.PKAchu AKAREV Balb/c-Tg(AKAR3EV)pT2A-3901NES nbio_283 Ubiquitous
PKA lox-PKAchu AKAREV B6-Tg(AKAR3EV)pT2A-lox-tdKeima-3901NES nbio_208 Cre-inducible Goto 2015; Yamaguchi 2015; Genotyping
PKA Balb/c.lox-tdKeima-PKAchu AKAREV Balb/c-Tg(AKAR3EV)pT2A-lox-tdKeima-3901NES nbio_285 Cre-inducible
PKA B6 Albino-PKAchu, hairless AKAREV B6 albino, hairless-Tg(AKAR3EV)pT2A-3901NES nbio_318 Ubiquitous Konagaya 2017; Sato 2020
PKA B6 Albino-lox-tdKeima-PKAchu AKAREV B6 albino-Tg(AKAR3EV)pT2A-lox-tdKeima-3901NES nbio_320 Cre-inducible
AMPK lox-AMPK AMPKAREV B6.Tg(lox-Eevee-AMPK)pT2A-lox-tdKeima-4031NES nbio_268 Cre-inducible Konagaya 2017
AMPK AMPAM AMPKAREV B6.Tg(AMPK)pT2A-4031NES nbio_275 Ubiquitous Konagaya 2017
AMPK AMPKAREV B6-Tg(hyBRET-AMPK)pT2A-6034NES nbio_327 Ubiquitous
S6K lox-S6K Eevee-S6K B6.Tg(lox-Eevee-S6K)pT2A-lox-tdKeima-3568NES nbio_269 Cre-inducible
S6K hyBRET-S6K B6-Tg(hyBRET-S6K)pT2A-6015NES nbio_328 Ubiquitous
TAK1 hyBRET-TAK1 B6-Tg(hyBRET-TAK1)pT2A-6035NES nbio_329 Ubiquitous
CrkII lox-Picchu-B6 Picchu B6C3F1-Tg(pT2A-lox-tdKeima-Picchu-734)#1 nbio_221 Cre-inducible
CrkII lox-Picchu-FVB Picchu (FVBxB6C3F1)F1-Tg(pT2A-lox-tdKeima-Picchu-734)#1 nbio_222 Cre-inducible
CrkII lox-Picchu-ICR Picchu (ICRxB6C3F1)F1-Tg(pT2A-lox-tdKeima-Picchu-734)#2 nbio_223 Cre-inducible
Negative control PKAchu-NC AKAREV-NC ICR~(ICR~B6C3F1)N2-Tg(pT2A-3906NES)#2 nbio_186 Ubiquitous Kamioka 2012; Sato 2020; AKAREV without phosphorylation site
Negative control B6 Albino-PKAchu-NC AKAREV-NC:Albino hairless B6 Albino-Tg(pT2A-3906NES) nbio_319 Ubiquitous Konagaya 2017; Sato 2020; Genotyping
Low molecular weight GTPases
Cdc42 Raichu-Cdc42 B6.Tg(Raichu-Cdc42)pT2A-Raichu-2253x nbio_191 Ubiquitous Goto 2013
Rac1 Raichu-Rac1 (B6~B6C3F1)F1-Tg(pT2A-lox-tdKeima-Raichu-2246x)#1 Ubiquitous Goto 2013
Ras FVB.lox-HRas Raichu-HRas FVB-Tg(lox-Raichu-HRas)pT2A-lox-tdKeima-Raichu-3715x nbio_218 Cre-inducible
Phosphoinositides
PI3,4P2 Pippi-PI3,4P2 (ICR~B6C3F1)F1-Tg(pT2A-1759xRe) nbio_191 Ubiquitous